Use of dabsylation, column switching and chiral separation for the determination of a renin inhibitor in rat, marmoset and human plasma
Summary
Type: HPLC Application #: 107880
Catalog#: 720071.40 - MN HPLC Column, Nucleosil C8, 120A, 3um, 4x125mm
Detection: UV-VIS, 478nm, 0.005 AUFS after derivatization with 4-dimethylaminoazobenzene-4'-sulphonyl (dabsyl) chloride Conditions: eluent: acetonitrile - 100mM Na acetate buffer pH 3.5 containing 7mM b-cyclodextrin flow rate 1.5ml/min Authors: Ba, B. et al. Source: J. Chromatogr. 572 (1991) 277–289
Details
Legend: Fig. 4. Chromatograms from A) human control plasma, B) rat control plasma, C)
marmoset control plasma, D) rat plasma spiked with 5 ng/ml Ro 42-5892/001 (I)
and 100 ng internal standard (II), E) rat